Samuel Estabrooks assists the firm in biotechnology patent prosecution. Sam has extensive knowledge in the areas of molecular biology and biochemistry. His experience also spans the areas of in vitro assay development, protein purification, cloning, human cell culture, protein radiolabeling, radiography and immunoblotting.

Prior to joining Wolf Greenfield, Sam received his PhD in Biology from the University of Pittsburgh, where his doctoral studies were concentrated in molecular, cellular and developmental biology. As a graduate student researcher in Jeffrey L. Brodsky’s lab, he developed novel assays which he applied to identify subtle differences in the way that different allelic variants of cystic fibrosis are recognized and processed by cells.

  • 2017 Cystic Fibrosis Foundation Student Traineeship


  • Estabrooks, S.K., Brodsky, J.L. “Ubiquitination of disease-causing CFTR variants in a microsome-based assay” Anal Biochem 2020, 604:113829.
  • Estabrooks, S., Brodsky, J.L. “Regulation of CFTR biogenesis by the proteostatic network and pharmacological modulators” Int J Mol Sci 2020, 21(2), 452. doi:10.3390/ijms21020452
  • Zaman, K., Knight, J., Hussain, F., Cao, R., Estabrooks, S.K., Altawallbeh, G., Holloway, K., Jafri, A., Sawczak, V., Li, Y., et al. “S-Nitrosylation of CHIP enhances F508del-CFTR maturation” Am J Respir Cell Mol Biol 2019, 61(6), 765-775. doi:10.1165/rcmb.2018-0314OC
  • Werner, H.M., Estabrooks, S.K., Preston, G.M., Brodsky, J.L., Horne, W.S. “Exploring the functional consequences of protein backbone alteration in ubiquitin through native chemical ligation” Chembiochem 2019, 20(18), 2346-2350. doi:10.1002/cbic.201900225
  • Pabon, N.A., Xia, Y., Estabrooks, S.K., Ye, Z., Herbrand, A.K., Süß, E., Biondi, R.M., Assimon, V.A., Gestwicki, J.E., Brodsky, J.L., Camacho, C.J., Bar-Joseph, Z. “Predicting protein targets for drug-like compounds using transcriptomics” PLoS Comput Biol 2018, 12(24), e1006651. doi:10.1371/journal.pcbi.1006651
  • Ye, Z., Needham, P.G., Estabrooks, S.K., Whitaker, S.K., Garcia, B.L., Misra, S., Brodsky, J.L., Camacho, C.J. “Symmetry breaking during homodimeric assembly activates an E3 ubiquitin ligase” Sci Rep 2017, 7(1)1789. doi:10.1038/s41598-017-01880-4

In his free time, Sam enjoys hiking, kayaking, canoeing and gardening, as well as software modding, 3D modeling and other digital arts.
Expand All

Samuel Estabrooks assists the firm in biotechnology patent prosecution. Sam has extensive knowledge in the areas of molecular biology and biochemistry. His experience also spans the areas of in vitro assay development, protein purification, cloning, human cell culture, protein radiolabeling, radiography and immunoblotting.

Prior to joining Wolf Greenfield, Sam received his PhD in Biology from the University of Pittsburgh, where his doctoral studies were concentrated in molecular, cellular and developmental biology. As a graduate student researcher in Jeffrey L. Brodsky’s lab, he developed novel assays which he applied to identify subtle differences in the way that different allelic variants of cystic fibrosis are recognized and processed by cells.

  • 2017 Cystic Fibrosis Foundation Student Traineeship
  • Estabrooks, S.K., Brodsky, J.L. “Ubiquitination of disease-causing CFTR variants in a microsome-based assay” Anal Biochem 2020, 604:113829.
  • Estabrooks, S., Brodsky, J.L. “Regulation of CFTR biogenesis by the proteostatic network and pharmacological modulators” Int J Mol Sci 2020, 21(2), 452. doi:10.3390/ijms21020452
  • Zaman, K., Knight, J., Hussain, F., Cao, R., Estabrooks, S.K., Altawallbeh, G., Holloway, K., Jafri, A., Sawczak, V., Li, Y., et al. “S-Nitrosylation of CHIP enhances F508del-CFTR maturation” Am J Respir Cell Mol Biol 2019, 61(6), 765-775. doi:10.1165/rcmb.2018-0314OC
  • Werner, H.M., Estabrooks, S.K., Preston, G.M., Brodsky, J.L., Horne, W.S. “Exploring the functional consequences of protein backbone alteration in ubiquitin through native chemical ligation” Chembiochem 2019, 20(18), 2346-2350. doi:10.1002/cbic.201900225
  • Pabon, N.A., Xia, Y., Estabrooks, S.K., Ye, Z., Herbrand, A.K., Süß, E., Biondi, R.M., Assimon, V.A., Gestwicki, J.E., Brodsky, J.L., Camacho, C.J., Bar-Joseph, Z. “Predicting protein targets for drug-like compounds using transcriptomics” PLoS Comput Biol 2018, 12(24), e1006651. doi:10.1371/journal.pcbi.1006651
  • Ye, Z., Needham, P.G., Estabrooks, S.K., Whitaker, S.K., Garcia, B.L., Misra, S., Brodsky, J.L., Camacho, C.J. “Symmetry breaking during homodimeric assembly activates an E3 ubiquitin ligase” Sci Rep 2017, 7(1)1789. doi:10.1038/s41598-017-01880-4


In his free time, Sam enjoys hiking, kayaking, canoeing and gardening, as well as software modding, 3D modeling and other digital arts.